Treatment of Diesel Oil Contaminated Soil by Ex-situ Bioremediation


  • N. Rajamohan Department of Chemical Engineering, Sohar University, Oman
  • R. Manivasagan Department of Chemical Engineering, Annamalai University, India
  • F. Al Fazari Faculty of Engineering, Sohar University, Oman
Volume: 9 | Issue: 4 | Pages: 4334-4337 | August 2019 |


Treatment of oil-polluted soil is a challenging problem faced by all refineries and petrochemical industries. In this research study, bioremediation of diesel oil contaminated soil was conducted for diesel concentration ranging from 5% to 20%. The physicochemical characteristics of diesel oil contaminated soil were studied. The effects of soil amendments, namely coconut ash powder, biofilter activated sludge, and NPK fertilizer, on total petroleum hydrocarbon removal efficiency were studied. The maximum total petroleum hydrocarbon removal efficiency achieved was 94.5% when 4g NPK, 40g of activated sludge and 40g of coconut ash powder per 1000g of contaminated soil were used. The studies on the effect of temperature confirmed the optimal temperature as 35°C. The parametric studies confirmed that the degradation efficiency decreased with increase in diesel oil concentration.


bioremediation, fertilizer, sludge, diesel, efficiency


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M. Gavrilescu, K. Demnerova, J. Aamand, S. Agathos, F. Fava, “Emerging pollutants in the environment: present and future challenges in biomonitoring, ecological risks and bioremediation”, New Biotechnology,Vol. 32, No. 1, pp. 147-156, 2015 DOI:

B. Sharma, A.K. Dangi, P. Shukla, “Contemporary enzyme based technologies for bioremediation: A review”, Journal of Environmental Management, Vol. 210, pp. 10-22, 2018 DOI:

S. Rayu, D.G. Karpouzas, B.K. Singh, “Emerging technologies in bioremediation: constraints and opportunities”, Biodegradation, Vol. 23, No. 6, pp 917-926, 2012 DOI:

N. Rajamohan , J. Al-Sinani ,V. Saravanan, M. Rajasimman, “Biodegradation of ethyl benzene and xylene contaminated air in an up flow mixed culture bio filter”, International Biodeterioration & Biodegradation, Vol. 119, No.C, pp. 309-315, 2017 DOI:

K. Urum, T. Pekdemir and M. Copur, “Surfactants treatment of diesel oil contaminated soils”, Journal of Colloid and Interface Science, Vol. 276, No. 2, pp. 456-464, 2004 DOI:

F.A. Orji, A.A. Ibiene, E.N. Dike, “Laboratory Scale Bioremediation of Petroleum Hydrocarbon – Polluted Mangrove Swamps in the Niger Delta Using Cow Dung”, Malaysian Journal of Microbiology, Vol. 8, No. 4, pp. 226-235, 2012 DOI:

M.J. Ayotamuno, R.B. Kogbara, S.O.T. Ogajib, S.D. Probert, “Bioremediation of a diesel oil polluted agricultural-soil at Port Harcourt” , Applied Energy, Vol. 83, No. 11, pp. 1249-1257, 2006 DOI:

Y. Wang, J. Feng, Q. Lin, “Effects of diesel oil contamination on soil physical and chemical properties”, Chinese Geographical Science, Vol. 23, No. 6, pp 708–715, 2013 DOI:

Mrayyan , M.N. Battikhi , “Biodegradation of total organic carbons (TOC) in Jordanian petroleum sludge”, Journal of Hazardous Materials, Vol. 120, No. 1-3, pp. 127-134, 2005 DOI:

R.R. Bruce, A. Klute, “The Measurement of Soil Moisture Diffusivity”, Soil Science Society of America Journal, Vol. 20, No. 4, pp. 458-462, 1955 DOI:

N. Das, P. Chandran, “Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview”, Biotechnology Research International, pp. 1-13, 2011 DOI:

R.F. Hesnawi, F.S. Mogadami, “Bioremediation of crude oil-contaminated soil under Mesophilic and Thermophilic conditions”, APCBEE Procedia, Vol. 5, pp. 82-87, 2013 DOI:

H. Chaîneau, C. Yepremian, J. F. Vidalie, J. Ducreux, D. Ballerini, “Bioremediation of a Diesel Oil-Polluted Soil: Biodegradation, Leaching and Toxicity Assessments”, Water, Air & Soil Pollution, Vol. 144, No. 1-4, pp. 1-419, 2003 DOI:

M. Chorom, H. S. Sharifi, H. Motamedi, “Bioremediation; Fertilizer; Heterotrophic bacteria; Diesel oil; Normal paraffin”, Iranian Association of Environmental Health (IAEH), Vol. 7, No. 3, pp. 319-326, 2011

X. U. Yaohui, L.U. Mang, “Bioremediation of diesel oil-contaminated soil: Comparison of different bio stimulation and bio augmentation treatments”, Journal of Hazardous Materials, Vol. 183, No.1-3,pp.395-401, 2010 DOI:

Mrozika , Z. Piotrowska-Segetb, “Bioaugmentation as a strategy for cleaning up of soils contaminated with aromatic compound”, Microbiological Research, Vol. 165, No. 5, 363-375, 2010 DOI:

S. Dimitrov, T. Pavlov, D. Nedelcheva, P. Reuschenbach, M. Silvani, R. Bias, “A kinetic model for predicting biodegradation”, SAR and QSAR in Environmental Research, Vol. 18, No. 5-6, pp. 443-57, 2007 DOI:

O.P. Abioye, P. Agamuthu , A. R. Abdul Aziz ,”Biodegradation of Used Motor Oil in Soil Using Organic Waste Amendments”, . Biotechnology Research International, pp. 1-8, 2012 DOI:

A. Burghal, N.A. Al-Mudaffar ,K.H. Mahdi, “Ex situ bioremediation of soil contaminated with diesel oil by use of actinomycetes consortia for process bio augmentation”, European Journal of Experimental Biology, vol. 5, pp.24-30, 2015


How to Cite

N. Rajamohan, R. Manivasagan, and F. Al Fazari, “Treatment of Diesel Oil Contaminated Soil by Ex-situ Bioremediation”, Eng. Technol. Appl. Sci. Res., vol. 9, no. 4, pp. 4334–4337, Aug. 2019.


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