Silver Nanoparticle-Modified Screen-Printed Carbon Electrodes for the Electrochemical Sensing of the Prostate-Specific Antigen
Received: 9 July 2025 | Revised: 25 October 2025 and 24 November 2025 | Accepted: 26 November 2025 | Online: 24 December 2025
Corresponding author: Yahia F. Makableh
Abstract
This study focuses on the development of an electrochemical aptamer-based biosensor based on silver Nanoparticle (AgNP)-modified Screen-Printed Carbon Electrodes (SPCEs) and its sensing potential against Prostate-Specific Antigen (PSA), a primary biomarker of Prostate Cancer (PCa). A significant challenge in conventional PCa screening methods is their inability to detect PSA with high sensitivity in early cancer stages. SPCEs modified with nanomaterials can overcome this limitation and revolutionize cancer diagnosis. The electrochemical behavior of the proposed biosensor was studied using voltametric and impedimetric methods. Atomic Force Microscopy (AFM) and Fourier Transform Infrared spectroscopy (FTIR) were also utilized to confirm the assembly of the sensor. The Limit of Detection (LOD) was assessed by deploying differential pulse voltammetry, where the sensor demonstrated high sensitivity and selectivity towards PSA. The LOD of the biosensor was 0.004 ng/ml, which underscores its potential as a robust tool for early PCa detection in point-of-care settings.
Keywords:
aptasensor, PSA detection, electrochemical detection, silver nanoparticlesDownloads
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Copyright (c) 2025 Yahia F. Makableh, Tamara Athamneh, Rama Matar, Ahmad Abu-Baker, Sara Hijazi, Aws Al-Qaisi
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