Rapid Quantitative Detection of Cannabinoids using Laser Raman Spectroscopy

Authors

  • Oranat Chuchuen Department of Chemical Engineering and Biomedical Engineering Program, Faculty of Engineering, Khon Kaen University, Khon Kaen, Thailand | Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand
  • Rungtip Madee Research and International Relations Affairs, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
  • Jakkapat Paluka Cannabis Research Institute, Khon Kaen University, Khon Kaen, Thailand
  • Chanon Lapjit Department of Horticulture, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand | Cannabis Planting and Extraction Center, Khon Kaen University, Khon Kaen, Thailand
  • Pewpan M. Intapan Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand
Volume: 14 | Issue: 5 | Pages: 16998-17004 | October 2024 | https://doi.org/10.48084/etasr.8203

Abstract

The current gold-standard methods for cannabinoid measurement are highly sensitive, yet expensive, sophisticated, and time-consuming. This study investigated the potential of laser Raman spectroscopy as a rapid and straightforward method for the quantitative detection of three major cannabinoids: Cannabidiol (CBD), Cannabinol (CBN), and Delta-9-tetrahydrocannabinol (THC). A series of solutions of the cannabinoids extracted from Cannabis sativa were prepared in Tetrahydrofuran (THF) and measured with a Near-Infrared (NIR)-excited Raman microspectrometer. The Raman spectra were subjected to chemometrics-based multivariate analysis, employing an ordinary least square fitting method. A strong linear relationship (R² > 0.98) was observed between the Raman intensity and the concentration of all studied cannabinoids. A Raman-based prediction model of each cannabinoid was developed based on a leave-one-out cross-validation analysis, which yielded a minimum detectable concentration in the range of 0.23-0.42 mg/ml, as determined by the Root Mean Square Error of Cross Validation (RMSECV). Additionally, characteristic Raman marker bands for each cannabinoid were identified. Overall, this study presented a simple yet effective Raman spectroscopy-based technique for rapid label-free cannabinoid detection and measurement.

Keywords:

cannabidiol, cannabinol, delta-9-tetrahydrocannabinol, Raman spectroscopy, optical spectrometry

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How to Cite

[1]
Chuchuen, O., Madee, R., Paluka, J., Lapjit, C. and Intapan, P.M. 2024. Rapid Quantitative Detection of Cannabinoids using Laser Raman Spectroscopy. Engineering, Technology & Applied Science Research. 14, 5 (Oct. 2024), 16998–17004. DOI:https://doi.org/10.48084/etasr.8203.

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