Isolation of Crude Oil from Polluted Waters Using Biosurfactants Pseudomonas Bacteria: Assessment of Bacteria Concentration Effects

A. Khalifeh, B. Roozbehani, A. M. Moradi, S. Imani Moqadam, M. Mirdrikvand

Abstract


Biological decomposition techniques and isolation of environmental pollutions using biosurfactants bacteria are effective methods of environmental protection. Surfactants are amphiphilic compounds that are produced by local microorganisms and are able to reduce the surface and the stresses between surfaces. As a result, they will increase solubility, biological activity, and environmental decomposition of organic compounds. This study analyzes the effects of biosurfactants on crude oil recovery and its isolation using pseudomonas sea bacteria species. Preparation of biosurfactants was done in glass flasks and laboratory conditions. Experiments were carried out to obtain the best concentration of biosurfactants for isolating oil from water and destroying oil-in-water or water-in-oil emulsions in two pH ranges and four saline solutions of different concentrations. The most effective results were gained when a concentration of 0.1% biosurfactants was applied.


Keywords


environmental decomposition; biological separation; biosurfactant; pseudomonas; concentration

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References


G. H. Ebrahimipur, J. Fuladi, A. Ferdosi, “Environmental factors’ effect assessment on crude oil separation via extreme haluphil oil eater bacteria producing biosurfactant Pars Q2 and Gravimeter of oil cuts consumed by these bacteria in optimum conditions”, Natural Science Journal, Vol. 13, pp. 59-70, 2005 (in Persian)

G. H. Ebrahimipur, J. Fuladi, A. Ferdosi, “Separation and specification of RK extreme haluphil oil eater bacteria bearing biosurfactant and assessment of salt concentration amount on crude oil separation via this “suyeh””, Natural Science Journal, Vol. 12, pp. 9-20, 2004 (in Persian)

A. Singh, J. D. Van Hamme, O. P. Ward, “Surfactants in microbiology and biotechnology: Part 2. Application aspects”, Biotechnology Advances, Vol. 25, No. 1, pp. 99-121, 2007

M. P. Plociniczak, G. A. Plaza, Z. Piotrowska-Seget, S. S. Cameotra, “Environmental Application of Biosurfactants: Recent Advances", International Journal of Molecular Sciences, Vol. 12, No. 1, pp. 633-654, 2011

N. K. Bordoloi, B. K. Onwar, "Microbial surfactant-enhanced mineral oil recovery under laboratory condition", Colloids and Surfaces, Vol. 63, No. 1, pp. 73-82, 2008

R. Thavasi, S. Jayalakshmi, I. M. Banat, " Effect of biosurfactant and fertilizer on biodegradation of crude oil by marine isolates of Bacillus megaterium, Corynebacterium kutscheri and Pseudomonas aeruginosa", Bioresource Technology, Vol. 102, No. 2, pp. 772-778, 2011

R. Thavasi, S. Jayalakshmi, T. Balasubramanian, I. M. Banat, "Production and characterization of a glycolipid biosurfactant from Bacillus megaterium using economically cheaper sources", World Journal of Microbiology and Biotechnology, Vol. 24, No. 7, pp. 917-925, 2008

Z. A. Raza, Z. M. Khalid, I. M. Banat, "Characterization of rhamnolipids produced by a Pseudomonas aeruginosa mutant strain grown on waste oils", Journal of Environmental Science and Health, Vol. 44, No. 13, pp. 1367-1373, 2009

B. Kumari, S.N. Singh, D.P. Singh, “Characterization of two biosurfactant producing strains in crude oil degradation”, Process Biochemistry, Vol. 47, No. 12, pp. 2463–2471, 2012




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